INFLUENCE OF OZONE ON THE CULTURE OF MERISTEMS
Relatively much harm in agriculture is caused by various harmful organisms, including viruses, living in plants and frequently reducing the volume and quality of crop. Therefore, production of healthy planting stock is one of the most important preconditions. Culture of meristems is often used in pomiculture, in order to obtain healthy planting stock, i. e., in tubes are grown microplants, obtained from the tissue of sprout and bud crowns. However, this method is not always efficient enough for elimination of viruses in plants. Other methods, such as thermotherapy, chemotherapy and influence of other physical factors, are also used for revitalization of such plants.
Being aware of the destructive influence of ozone on C-C bonds, we decided to test its influence on microplants. In 2010-2011, tests were conducted in Pūre Horticulture Research Center, in order to establish the influence of ozone on raspberry microplants and elimination of RBDV in them.
Evaluation of the results showed that microplants are almost being killed by ozone dose of 0.25 ppm. However crown tissue of 95 % of microplants remained live, and plants grew from them. After performance of plant virus test it was detected that 25 % of plants were virus-free. Search for optimum method of ozone use should be further continued, but the obtained results indicate that use of ozone for virus elimination in microplants is possible.
For more detailed information please see the PDF:
L. Lepse, 2011. Influence of ozone on in vitro plants. Advances in plant biotechnology in Baltic sea region. March 30-31, Kaunas, Lithuania. 55-57 p.